RESUMEN
The emergence of bacterial species is rooted in their inherent potential for continuous evolution and adaptation to an ever-changing ecological landscape. The adaptive capacity of most species frequently resides within the repertoire of genes encoding the secreted proteome (SP), as it serves as a primary interface used to regulate survival/reproduction strategies. Here, by applying evolutionary genomics approaches to metagenomics data, we show that abundant freshwater bacteria exhibit biphasic adaptation states linked to the eco-evolutionary processes governing their genome sizes. While species with average to large genomes adhere to the dominant paradigm of evolution through niche adaptation by reducing the evolutionary pressure on their SPs (via the augmentation of functionally redundant genes that buffer mutational fitness loss) and increasing the phylogenetic distance of recombination events, most of the genome-reduced species exhibit a nonconforming state. In contrast, their SPs reflect a combination of low functional redundancy and high selection pressure, resulting in significantly higher levels of conservation and invariance. Our findings indicate that although niche adaptation is the principal mechanism driving speciation, freshwater genome-reduced bacteria often experience extended periods of adaptive stasis. Understanding the adaptive state of microbial species will lead to a better comprehension of their spatiotemporal dynamics, biogeography, and resilience to global change.
Asunto(s)
Adaptación Fisiológica , Bacterias , Agua Dulce , Genoma Bacteriano , Filogenia , Bacterias/genética , Bacterias/clasificación , Agua Dulce/microbiología , Adaptación Fisiológica/genética , Metagenómica/métodos , Evolución Molecular , Tamaño del Genoma , Proteoma/genética , Proteoma/metabolismoRESUMEN
This study reports the complete genome of Flavobacterium pectinovorum str. ZE23VCel01 isolated from a freshwater environment. By means of Nanopore Q20+ chemistry, the chromosome was assembled as a circular element with a length of 5.9 Mbp, a GC content of 33.58%, and a coverage of 122×.
RESUMEN
Here, we report the complete genome of Pseudomonas kielensis str. Ze23jcel16 isolated from a freshwater sample. The high-quality chromosome was obtained employing R10.4.1 Nanopore Flow cell chemistry and was assembled as a circular element at 45× coverage, a length of 5.8 Mbp, and a G+C content of 61.15%.
RESUMEN
We present here the complete genome of Asticcacaulis sp. ZE23SCel15. The strain was isolated from the surface water of Lake Zurich, Switzerland. The assembly of high-quality Q20+ Nanopore data yielded a circular genome with ~3.8 Mb (coverage: 34×) and a GC content of 56.81%.
RESUMEN
We report here a complete metagenome-assembled genome belonging to the AKYH767 order within the Bacteroidota phylum. The recovered genome stems from a nonaxenic Amoebozoa culture from Lake Zürich and was assembled as a circular element at a length of 4.1 Mbp and a coverage of 115×.
RESUMEN
Here, we report the complete metagenome-assembled genome of an uncultivated freshwater Flavobacterium sp. recovered from a nonaxenic Amoebozoa sp. culture. The chromosome was obtained from a metagenomic long-read sequencing run and was assembled as a circular element at a 51× coverage, length of 3.8 Mbp and a G+C content of 37.37%.
RESUMEN
We report here a complete metagenome-assembled genome sequence belonging to the Proteobacteria phylum within the Hyphomonadaceae family. The circular chromosome was obtained from a metagenomic long-read sequencing run and displays a length of 4.1 Mbp along with a GC content of 62.5% at a coverage of 81×.
RESUMEN
Here, we report the first complete genome of an uncultivated freshwater Elusimicrobiota organism recovered from a nonaxenic Amoebozoa sp. culture. The chromosome was obtained from a metagenomic long-read sequencing run and was assembled as a circular element at a 47× coverage, a length of 3.8 Mbp, and a G+C content of 68.6%.
